wako 125-05061賴氨酰肽鏈內切酶 Lysyl Endopeptidase 
【產品詳情】
賴氨酰肽鏈內切酶
品牌：WAKO 和光
級別：質譜級

英文品名：Lysyl Endopeptidase?, Mass Spectrometry Grade
貨號：125-05061
規格：5×20 μg
應用：蛋白質組學。
用途：賴氨酰肽鏈內切酶，蛋白測序、質譜分析，多肽合成

【實驗原理】
賴氨酰肽鏈內切酶是質譜分析前處理時常用的蛋白分解酶即賴氨酰肽鏈內切酶，賴氨酰肽鏈內切酶可以特異性切除賴氨酸基團C 末端的多肽，賴氨酰肽鏈內切酶可用于蛋白測序分析和Lys-X 化合物的酶合成。
若同時使用賴氨酰肽鏈內切酶和胰酶，可更好地切斷賴氨酸基團的多肽，增加多肽的數量。賴氨酰肽鏈內切酶已按照使用習慣做成小包裝，是方便使用賴氨酰肽鏈內切酶的冷凍干燥品。

【產品特點】
賴氨酰肽鏈內切酶Lysyl Endopeptidase 125-05061 wako
1）賴氨酰肽鏈內切酶用于蛋白質譜分析：高特異性、高蛋白質消化率；
2）賴氨酰肽鏈內切酶能增加肽段數量、提高裂解效率；
3）賴氨酰肽鏈內切酶獨立包裝，20 μg/一支

【結果】表明Lep 酶解的錯誤裂解率低。
Tp 酶解時加入Lep 后，錯誤裂解率有所降低，同時，賴氨酰肽鏈內切酶可以鑒定出更多的多肽。

賴氨酰肽鏈內切酶Lysyl Endopeptidase 125-05061 wako
Lep+Tp 賴氨酰肽鏈內切酶切后，可以在m/z=2000 時得到吸收峰，而單獨的Tp 酶切在m/z=2000 時沒有吸收峰。該結果表明Lep 可以提高測序覆蓋度。
賴氨酰肽鏈內切酶Lysyl Endopeptidase 125-05061 wako

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