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    畢赤酵母表達系統說明書

    發布時間:2013/8/9      點擊次數:2804

    畢赤酵母表達系統說明書

    You can put the power of P. pastoris into your laboratory with a complete Pichia Expression Kit or with individual Pichia expression vectors. Three Pichia Expression Kits are available, each of which includes vectors, P. pastoris strains, reagents for transformation, sequencing primers, media, and a comprehensive manual (Table 3). In addition, the pPICZ, pGAPZ, and pPIC6 vectors are available separay (see insert for ordering information). Choose the kit or vector that best meets your research needs and take advantage of the proven strength of P. pastoris.

    Table 3—Pichia expression kit components.

    EasySelect™ Pichia

    Expression Kit

    Multi-Copy Pichia

    Expression Kit

    Original Pichia

    Expression Kit

    Vectors

    pPICZ A, B, C 

    (20 µg each)

    pPICZα A, B, C 

    (20 µg each)

    pPIC9K (20 µg)

    pPIC3.5K (20 µg)

    pAO815 (20 µg)

    pPIC9 (10 µg) 

    pPIC3.5 (10 µg)

    pHIL-D2 (10 µg)

    pHIL-S1 (10 µg)

    Strains

    X-33 (Mut+

    , His+

    GS115 (Mut+

    KM71 (MutS

    GS115/pPICZ/lacZ 

    (control)

    GS115 (Mut+

    KM71 (MutS

    GS115/β-gal (control)

    GS115/albumin (control)

    GS115 (Mut+

    KM71 (MutS

    GS115/β-gal (control)

    GS115/albumin (control)

    Transformation 

    reagents

    Pichia EasyComp™ Kit

    (120 transformations)

    Spheroplast Module

    (50 transformations)

    Spheroplast Module

    (50 transformations)

    Sequencing

    primers

    5´ AOX1

    3´ AOX1

    α-factor

    5´ AOX1

    3´ AOX1

    α-factor

    5´ AOX1

    3´ AOX1

    α-factor

    Media and 

    supplements

    YP base medium 

     (2 pouches)*

    YP base agar 

     (2 pouches)*

    Yeast Nitrogen Base 

     (1 pouch)†

     Zeocin™ (250 mg)

    YP base medium 

     (2 pouches)*

    YP base agar

     (2 pouches)*

    Yeast Nitrogen Base 

     (1 pouch)†

    YP base medium 

     (2 pouches)*

    YP base agar

     (2 pouches)*

    Yeast Nitrogen Base

     (1 pouch)†

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